ATS 2020 Advance Program

3:50 Questions and Answers M. Cao, DO, Redwood City, CA CLINICAL • TRANSLATIONAL POSTGRADUATE COURSE PG17 A PRACTICAL, HANDS-ON INTRODUCTION TO GENOMIC ANALYSIS IN PULMONARY MEDICINE Pre-registration and additional fees required. Continental breakfast and box lunch included. Attendance is limited. Member: $350 In-Training Member: $200 Non-Member: $425 In-Training Non-Member: $300 Assemblies on Allergy, Immunology and Inflammation; Critical Care; Pediatrics; Respiratory Cell and Molecular Biology; Respiratory Structure and Function 8:00 a.m. - 4:00 p.m. Target Audience Pulmonary, pediatric pulmonology, sleep, and critical care physicians interested in better understanding the basis of genomic analysis. Physicians, providers, and researchers who want to acquire basic skills to design and conduct genomic studies Objectives At the conclusion of this session, the participant will be able to: • understand the importance of study design and data quality control when performing studies on the genomics of respiratory, sleep, and critical care medicine; • perform basic analyses of genome-wide association using microarray data (e.g. GWAS) and sequencing data (e.g. RNA-seq); • understand the principles behind single-cell RNA-sequencing and its implications for research in respiratory medicine. Our ability to study the human genome, including epigenomics, transcriptomics, and other “omics” has fueled a revolution in the study of disease. The field has continuously evolved at a fast pace, which can be intimidating. This hands-on course will help clinicians and investigators take their first steps into the field of genomics in respiratory medicine. We will cover the basics of study design and quality control, learn about the NHLBI’s new cloud-based DataSTAGE platform, and go through practical hands-on examples of GWAS, RNA-seq, single-cell RNA-seq, and pathway analysis. Chairing: E. Forno, MD, MPH, ATSF, Pittsburgh, PA B.E. Himes, PhD, Philadelphia, PA V.E. Ortega, MD, PhD, ATSF, Winston Salem, NC 8:00 Introduction E. Forno, MD, MPH, ATSF, Pittsburgh, PA 8:20 Study Design and Quality Control X. Li, PhD, Tucson, AZ 8:50 NHLBI TOPMed and DataSTAGE W. Gao, PhD, Bethesda, MD 9:10 DataSTAGE Practical Applications P. Avillach, PhD, MD, Boston, MA 9:30 Break 9:40 GWAS: Data Processing and Analysis Example D.G. Torgerson, PhD, Montreal, Canada 10:40 RNA-Seq: Alignment, QC, and Analysis M. Kan, PhD, Philadelphia, PA 11:40 LUNCH 12:35 RNA-Seq Part Two: Practical Analysis Examples B.E. Himes, PhD, Philadelphia, PA 1:35 Pathway Analysis I.V. Yang, BS, PhD, Aurora, CO 2:35 Break 2:45 Single-Cell RNA-Seq Demo and Examples W. Chen, PhD, Pittsburgh, PA 3:45 Conclusions V.E. Ortega, MD, PhD, ATSF, Winston Salem, NC ATS 2020 • Philadelphia, PA SATURDAY • MAY 16 21