ATS 2020 Advance Program

3:00 Case Presentations S. Sharma, MD, MPH, Aurora, CO S. Sahetya, MD, MHS, Baltimore, MD B. Coruh, MD, Seattle, WA I.S. Douglas, MD, Denver, CO BASIC • TRANSLATIONAL POSTGRADUATE COURSE PG9 A HANDS-ON INTRODUCTION TO STUDYING THE LUNG MICROBIOME Pre-registration and additional fees required. Continental breakfast and box lunch included. Attendance is limited. Member: $350 In-Training Member: $200 Non-Member: $425 In-Training Non-Member: $300 Assemblies on Pulmonary Infections and Tuberculosis; Allergy, Immunology and Inflammation; Section on Genetics and Genomics 8:00 a.m. - 4:00 p.m. Target Audience Research scientists interested in designing, performing, and understanding the analysis of lung and airway microbiota using sequencing-based techniques Objectives At the conclusion of this session, the participant will be able to: • understand research design and analytical approaches needed to conduct culture independent assessment of microbial communities in respiratory specimens; • integrate microbiota sequencing results with current culture-based models of respiratory infection. Audience will appreciate the complexity of re-evaluating our disease paradigms and apply this knowledge when designing studies of lung microbiota; • illustrate the use of a combination of basic and multi-dimensional computational approaches for investigating microbial function and mechanisms of disease. Audience will be equipped to prioritize mechanistic approaches to lung microbiota. This course will introduce methodologies used to study lung microbiota using culture-independent methods. Participants will learn how to design, execute, analyze and interpret a lung microbiome study. Hands-on experiences will include facilitated small group discussions on designing and executing lung microbiome studies, as well as demonstrations on the use of statistical software to analyze microbiome data. Participants will be challenged to develop their own study design and analytic plan for a hypothetical microbiome study, and will be provided both with sample microbiome data as well as executable, annotated code to make sense of it. They will be walked through each step in the didactic sessions, and faculty will provide hands-on support to assist. This code file will be easily adaptable by attendees for later use on their own microbiome data. No prior training is necessary on the part of trainees. Chairing: R.P. Dickson, MD, Ann Arbor, MI A. Pragman, MD, PhD, Minneapolis, MN L.N. Segal, MD, New York, NY 8:00 An Introduction to Thinking About the Microbiome R.P. Dickson, MD, Ann Arbor, MI 8:50 Integrating Microbiota Findings with Our Disease Models A. Pragman, MD, PhD, Minneapolis, MN 9:20 Hands-on Breakout Session: Study Design 9:40 Studying Viruses and Fungi in Lung Microbiome Studies R.G. Collman, MD, Philadelphia, PA 10:10 Break 10:20 Contaminomics M.J. Cox, BSc(Hons), PhD, London, United Kingdom 10:50 Hands-OnBreakoutSession:SamplingandControls 11:10 Hands-On: A Crash Course in Microbiome Analysis: Part 1 C. Brown, BS, Ann Arbor, MI 11:50 LUNCH 12:50 Hands-On: A Crash Course in Microbiome Analysis: Part 2 C. Brown, BS, Ann Arbor, MI ATS 2020 • Philadelphia, PA 10 FRIDAY • MAY 15