ATS 2019 Virtual Final Program

BASIC • TRANSLATIONAL POSTGRADUATE COURSE CME Credits Available: 6.5 PG23 HIGH-CONTENT SINGLE-CELL TECHNIQUES TO STUDY LUNG IN HEALTH AND DISEASE R Pre-registration and additional fees required. Continental breakfast and box lunch included. Attendance is limited. Member: $350 In-Training Member: $200 Non-Member: $425 In-Training Non-Member: $300 : Registrants must bring a laptop to the course to view the course material. Assemblies on Respiratory Cell and Molecular Biology; Allergy, Immunology and Inflammation 8:00 a.m. - 4:00 p.m. KBHCCD Room D166 (Level1) Target Audience Basic and clinical researchers, postdocs, graduate students, clinical fellows Objectives At the conclusion of this session, the participant will be able to: • learn about the available high content single-cell technologies and their applicability to study human lung in health and disease; • learn about the bioinformatics and computational tools and resources available for analysis of high-content single-cell data in the context of the lung biology and disease; • design high content single-cell studies in human subjects. This postgraduate course will provide an introduction to the high content single cell techniques in the context of the lung development, aging, and disease. We will focus on single-cell RNA-seq (including CITE-seq and single nucleus RNA-seq), single-cell ATAC-seq and high content imaging (such as PLISH and MERFISH) and corresponding bioiformatics tools. Chairing: S. Herold, MD, PhD, Giessen, Germany M.C. Nawijn, PhD, Groningen, Netherlands A. Misharin, MD, PhD, Chicago, IL 8:00 Welcome S. Herold, MD, PhD, Giessen, Germany 8:05 Overview of the Single Cell scRNA-seq Methods H.B. Schiller, PhD, Neuherberg, Germany 8:40 Computational Tools for Single Cell Transcriptomic Analysis P. Reyfman, MD, Chicago, IL 9:15 Single-Cell Profiling of the Open Chromatin via HTS-seq T.E. Duong, MD, San Diego, CA 9:50 Break 10:00 Single-Nucleus RNA-seq to Study Archived Lung Samples A. Misharin, MD, PhD, Chicago, IL 10:35 Cell Atlas of the Human Lung V1.0 M.C. Nawijn, PhD, Groningen, Netherlands 11:10 Single Cell Proteomics in the Lung C. Ansong, PhD, Richland, WA 11:45 LUNCH 12:40 Analysis of High-Dimensional Cytometry Data A. Belkina, MD, PhD, Boston, MA 1:15 Spatial Transcriptomics to Understand Lung Development and Disease T. Desai, MD, MPH, Stanford, CA 1:50 Integrative Analysis of the Spatial and High-Throughput Single-Cell Transcriptomics V. Svensson, PhD, Pasadena, CA 2:25 Break 2:40 Light Sheet Microscopy for Studying Human Lung D. Shepherd, PhD, Aurora, CO 3:20 High-Content Automated Single Cell Imaging Analysis Using CellProfiler T. Becker, PhD, Cambridge, MA 3:55 Concluding Remarks M.C. Nawijn, PhD, Groningen, Netherlands CLINICAL POSTGRADUATE COURSE CME Credits Available: 7 PG24 PULMONARY FUNCTION TESTING IN THE REAL WORLD: 2019 UPDATES ON THE ART AND SCIENCE OF PFT R Pre-registration and additional fees required. Continental breakfast and box lunch included. Attendance is limited. Member: $350 In-Training Member: $200 Non-Member: $425 In-Training Non-Member: $300 : Registrants must bring a laptop to the course to view the course material. Assemblies on Respiratory Structure and Function; Clinical Problems 8:00 a.m. - 4:00 p.m. KBHCCD Room C144-C145 (Level 1) Target Audience Physicians, current/future PFT lab directors, respiratory therapists, trainees/fellows, and other interested healthcare providers. Basic knowledge of pulmonary physiology and PFT interpretation is desirable but not required Objectives At the conclusion of this session, the participant will be able to: • gain further understanding of the principles and practice of pulmonary function diagnostic tests; • develop greater confidence interpreting pulmonary function test results in clinical practice; • identify strategies to approach the interpretation of pulmonary function test results in complex patients. This course will focus on “real world” updates in lung function testing, combining guidelines from the new technical standards published in the past two years with hands-on experience in small group settings focusing on the performance, interpretation, and reporting of pulmonary function testing (PFT). We will combine ATS 2019 • Dallas, TX SATURDAY • MAY 18 21 SATURDAY