ATS 2019 Virtual Final Program

Target Audience Basic scientists, translational scientists Objectives At the conclusion of this session, the participant will be able to: • understand the conceptual approach of the human cell atlas, and its implications for lung health and disease; • gain knowledge of the cellular heterogeneity during lung development and in lung disease; • appreciate the power of single nuclear sequencing for retrospective clinical studies and spatial analyses. The human lung is a highly structured organ that contains at least 40 discrete cell types that contribute to its physiology and functions. To understand the lung and its diseases, we need to know the cells and their interactions that form this vital organ. Recent advances in single cell biology and spatial transcriptomics offer an unprecedented insight into the cellular and molecular architecture of the lung, the cell lineages that form its core structure and their interactions in health and disease. In this symposium, we will host a series of speakers that will provide a comprehensive overview of recent progress in charting an atlas of human lung cells, and its applications in understanding lung development, biomarker discovery and disease pathogenesis, as well as the cellular response to environmental insults. Chairing: G.H. Koppelman, MD, PhD, Groningen, Netherlands K.A. Steiling, MSc, MD, Boston, MA C.P. Hersh, MD, MPH, ATSF, Boston, MA 9:15 Introduction to Single Cell Sequencing of the Lung G.H. Koppelman, MD, PhD, Groningen, Netherlands 9:23 The Human Lung Cell Atlas: Charting Cellular Heterogeneity and Plasticity in Lung J. Rajagopal, MD, Boston, MA 9:39 Single Cell Transcriptomics in ARDS R. Zemans, MD, Ann Arbor, MI 9:55 Airway Epithelial Cell Heterogeneity Along the Respiratory Tract: Location, Location, Location P. Barbry, PhD, Valbonne, France 10:11 Single-Nuclear Sequencing of Human Lung Tissue: Empowering Spatial Analyses and Retrospective Analyses A. Misharin, MD, PhD, Chicago, IL 10:27 Unique Cell States and Interactions in Asthma: Applying the Human Lung Cell Atlas to Understand Disease Pathogenesis M.C. Nawijn, PhD, Groningen, Netherlands 10:43 Epigenetics of the Human Lung Cell Atlas: Single-Cell ATAC-Seq of Human Lung Tissue T.E.A. Duong, MD, San Diego, CA 10:59 Cigarette Smoking Induces Unique Cell States in Bronchial Epithelial Cells A. Spira, MD, Boston, MA BASIC • CLINICAL • TRANSLATIONAL SCIENTIFIC SYMPOSIUM CME Credits Available: 2 B11 LUNG-OMICS: TO BOLDLY GO... Assemblies on Pulmonary Circulation; Allergy, Immunology and Inflammation; Environmental, Occupational and Population Health; Nursing; Respiratory Cell and Molecular Biology; Section on Genetics and Genomics 9:15 a.m. - 11:15 a.m. KBHCCD Room D163/D165/D170/D172 (Level 1) Target Audience Physicians, nurses and researchers from any field who are interested in learning about state of the art omics approaches. Objectives At the conclusion of this session, the participant will be able to: • understand the different approaches used to interpret coding versus non-coding DNA variants and their functional relevance to disease; • learn how network analysis can be used to identify critical regulators in complex molecular interactions, and integrate clinical variables to develop risk stratification models for patients; • describe how ancestry and population structure impact the interpretation of genomic research and translation into the clinic. New omics technologies are providing unparalleled insight into the causes of lung disease and the potential to personalize therapy to specific endophenotypes, but they also present challenges for interpretation and clinical translation. This symposium showcases how omics approaches such as whole genome sequencing, epigenetics, proteomics and network analysis are being applied in lung diseases, with examples from pulmonary hypertension and asthma. The focus is on broad concepts more than specific pathways. We highlight the importance of considering ancestry in genomic studies to address health disparities, understand complex gene-environment interactions, and ensure that advances in precision medicine will benefit all populations. Chairing: M. Aldred, PhD, Indianapolis, IN K. Yuan, PhD, Palo Alto, CA S. Gräf, PhD, Cambridge, United Kingdom 9:15 Introduction M. Aldred, PhD, Indianapolis, IN 9:25 Whole Genome Sequencing: Challenges of Interpreting the Non-Coding Space S. Gräf, PhD, Cambridge, United Kingdom 9:47 Network Biology of the Regulatory Space S.S. Pullamsetti, PhD, Bad Nauheim Hessen, Germany 10:09 Network Biology of Non-Coding RNAs S.Y. Chan, MD, PhD, Pittsburgh, PA 10:31 Network Analysis in the Lab and the Clinic B.A. Maron, MD, Boston, MA 10:53 Translation to the Clinic: The Importance of Ancestry E.G. Burchard, MD, MPH, San Francisco, CA ATS 2019 • Dallas, TX 128 MONDAY • MAY 20